af594  (Bio-Rad)

Journal: Frontiers in Veterinary Science

Article Title: High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats

doi: 10.3389/fvets.2024.1498964

Figure Lengend Snippet: Canine immunophenotyping panel.

Article Snippet: CD5 , YKIX322.3 , AF594 , BioRad , MCA1037GA , N/A.

Techniques: Marker

Journal: Frontiers in Veterinary Science

Article Title: High-parameter immunophenotyping reveals distinct immune cell profiles in pruritic dogs and cats

doi: 10.3389/fvets.2024.1498964

Figure Lengend Snippet: Canine immunophenotyping panel.

Article Snippet: CD5 , YKIX322.3 , AF594 , BioRad , MCA1037GA , N/A.

Techniques: Marker

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Conjugated antibodies used for triple-labeling cells in acute leukemia immunophenotyping panels after April 2021, including their target antigen and registry number (when available) or source.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Conjugated antibodies used for triple-labeling cells in acute leukemia immunophenotyping panels after April 2021, including their target antigen and registry number (when available) or source.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Conjugated antibodies used for triple-labeling cells in a lymphoma immunophenotyping panel used for routine diagnostic testing after April 2021, including their target antigen*.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Diagnostic Assay

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Flow cytometric dot plots of anti-CD80 antibody labeling of leukocytes in blood from healthy dogs. (A) Three different cell populations were identified on a forward and side scatter plot, corresponding to neutrophils, monocytes, and lymphocytes. The cells were double-labeled with anti-CD80-APC and -CD14-PE antibodies, with CD14 being used as a monocyte marker. CD80 was only expressed on CD14 + monocytes and CD14 − neutrophils but not lymphocytes (CD80 − /CD14 − ). The few CD14 + and CD14 − cells in the neutrophil and monocyte gates likely represent low numbers of monocytes and neutrophils in the respective gates. The CD80 − /CD14 − cells in neutrophil and monocyte gates could be large lymphocytes (representative result from 1 of 4 dogs). (B) Triple-labeling of dog leukocytes with CD80-APC, CD21-PE and CD5-FITC shows that CD21 + B cells and CD5 + T cells are CD80 − (representative result from 1 of 3 dogs). All leukocyte events were combined for analysis vs. splitting the events into different gates based on forward and side scatter.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Antibody Labeling, Labeling, Marker

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Labeling of monocytes, B cells, T cells, and neutrophils isolated from the blood of healthy dogs (representative results from 1 of 2–3 dogs for each cell type) with the anti-CD80 antibody. (A-C) Monocytes, B cells, and T cells were isolated from peripheral blood mononuclear cells (PBMCs) using immunomagnetic beads and anti-CD14-PE, -CD21-FITC, and -CD5-FITC antibodies, respectively. The first panel shows the forward and side scatter events in PBMCs while the second panel shows the forward and side scatter events of isolated cells. The third panel is a fluorescent quadrant plot of double-labeled cells after adding the anti-CD80-APC antibody. The fourth panel shows a representative modified Wright’s-stained image of a cytospin smear of the isolated cells on which 100-cell differential cell counts were done (scale bar = 20 μm). (A) CD14-PE-isolated cells were mostly CD80 + monocytes (84% of a differential cell count), with a few contaminating neutrophils (10%) that were weakly CD14 + (arrows, third and fourth panels). A few monocytes had cytoplasmic vacuoles (fourth panel). Lymphocytes were negative for CD80 − /CD14 − (lower left quadrant, third panel, 6%). (B) CD21-FITC-isolated cells were mostly lymphocytes, which were CD80 − (third panel). Lymphocytes were primarily small cells, some of which had clefted or convoluted nuclei (variants of normal), with a few small or large reactive forms (fourth panel). (C) CD5-FITC-isolated cells were mostly lymphocytes, which were CD80 − (third panel). Lymphocytes were small cells with a few large or reactive forms. Several lymphocytes had a few clear cytoplasmic vacuoles, which could be due to the isolation procedure (fourth panel). (D) Neutrophils were isolated from the 1.077/1.119 interface of the double-density gradient used to obtain PBMCs and were single-labeled with the anti-CD80-APC antibody. The first panel shows a forward vs. side scatter plot of the isolated neutrophils and the second panel is a CD80 fluorescence vs. side scatter dot plot (blue) with overlaid hamster-APC isotype (red), showing neutrophils are CD80 + . The third panel shows a representative modified Wright’s-stained image of a cytospin smear of the isolated cells, which were primarily segmented neutrophils. The vacuolated cytoplasm in one neutrophil is likely an artifact of the isolation procedure (scale bar = 20 μm).

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Labeling, Isolation, Modification, Staining, Cell Counting, Fluorescence

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Percentage differential cell counts (mean and range) from modified Wright’s-stained cytospin smears of isolated monocytes, B cells, T cells, and neutrophils.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Modification, Isolation

Journal: Frontiers in Veterinary Science

Article Title: Flow cytometric-based detection of CD80 is a useful diagnostic marker of acute myeloid leukemia in dogs

doi: 10.3389/fvets.2024.1405297

Figure Lengend Snippet: Positive labeling with the anti-CD80 antibody in tumor cells in dogs with hematopoietic neoplasms.

Article Snippet: CD5-FITC/CD21-PE/CD45-APC , CD5 : Bio-Rad Cat# MCA1037F, RRID:AB_322643; CD21 : BD Biosciences Cat# 555422; RRID:AB_395816 CD45 : Bio-Rad Cat# MCA1042APC, RRID:AB_324810.

Techniques: Labeling

Journal: Veterinary Sciences

Article Title: Limited Clinical Efficacy with Potential Adverse Events in a Pilot Study of Autologous Adoptive Cell Therapy in Canine Oral Malignant Melanoma

doi: 10.3390/vetsci11040150

Figure Lengend Snippet: Representative flow cytometric results of the immunophenotype of the in vitro-expanded autologous infusion cells from four healthy donors indicate that the infusion cells were predominantly non-B non-T cells: ( a , c , e , g ) In donor Nos. 1 to 4, the cells were predominantly CD21 − CD5 − (85.1–88.5%), indicating a non-B phenotype; ( b , d , f , h ) In donor Nos. 1 to 4, the cells were predominantly CD3 − CD5 − (81.9–86.2%), indicating a non-T phenotype.

Article Snippet: The FITC-conjugated mouse anti-dog CD3, PE-conjugated rat anti-dog CD5, and Alex647-conjugated mouse anti-dog CD21 antibodies (all from Bio-Rad, Hercules, CA, USA) were used to stain the representative cell samples.

Techniques: In Vitro